abstract：:Formaldehyde vapor levels were evaluated in a histological fixative laboratory and environments where anatomical pieces treated with this fixative are stored or handled. Formaldehyde concentration in the air may reach values up to 9 times higher than that established (0.3 ppm) by the international official surveillanc...

journal_title：Journal of neuroscience methods

authors： Nogueira MI,Barbieri C,Vieira R,Marques ER,Moreno JE

更新日期：1997-03-01 00:00:00

abstract：:A discontinuous gradient of Percoll has been used to prepare a subcellular fraction (fraction III) from fish brain which is greatly enriched in synaptic nerve endings (synaptosomes). Synaptosomes display well-preserved morphology, good functional performance as assessed by ability to release preloaded neurotransmitter...

journal_title：Journal of neuroscience methods

authors： Nicholson RA,Zheng J

更新日期：1997-02-01 00:00:00

abstract：:The goal of the present study was an improvement of the widely used photoconversion technique, which still represents the major approach to the ultrastructural analysis of tissue labelled with fluorescent dyes. Since free access of oxygen to the tissue is essential for the dye-dependent photooxidation of diaminobenzid...

journal_title：Journal of neuroscience methods

authors： Kacza J,Härtig W,Seeger J

更新日期：1997-02-01 00:00:00

abstract：:The Thy-1.2 expression cassette described in this report drives strong constitutive expression of transgenes specifically in the neurons of adult transgenic mice. Transgene expression begins at P6-10, at a time when it can affect activity-dependent rearrangements of synaptic connections and neuron-glia interactions du...

journal_title：Journal of neuroscience methods

authors： Caroni P

更新日期：1997-01-01 00:00:00

abstract：:Several studies have reported on the use of primary neural cells transduced by adenoviral vectors as donor cells in neurotransplantation. In the present investigation, we examined whether adenoviral vector-mediated gene transfer could be used to introduce and express a foreign gene in solid neural transplants of fetal...

journal_title：Journal of neuroscience methods

authors： van Esseveldt KE,Liu R,Hermens WT,Verhaagen J,Boer GJ

更新日期：1997-01-01 00:00:00

abstract：:We describe a novel method for retrogradely labeling specific neuronal populations using voltage-sensitive dyes. Styryl dyes were injected into the ventral roots of the isolated embryonic chick spinal cord. After waiting several hours, the dye labeled motoneurons and autonomic preganglionic neurons. Neuronal cell bodi...

journal_title：Journal of neuroscience methods

authors： Wenner P,Tsau Y,Cohen LB,O'Donovan MJ,Dan Y

更新日期：1996-12-28 00:00:00

abstract：:We address three problems concerning the quantitative analysis of nerve cell distribution in the cerebral cortex: (i) preparatory tissue deformation (shrinkage); (ii) difficulties in differentiating between small neurons and astroglia; and (iii) the bias introduced by the counting method. We found that staining with R...

journal_title：Journal of neuroscience methods

authors： Skoglund TS,Pascher R,Berthold CH

更新日期：1996-12-28 00:00:00

abstract：:A new video path analyzer, described herein, monitors travel distance, rearing, stereotypic movement, rotation, and speed of rats simultaneously. The video path analyzer was equipped with a cage (black background, 50 cm long x 50 cm wide x 50 cm high), camera, television screen, computer, and printer. A detailed descr...

journal_title：Journal of neuroscience methods

authors： Pan WH,Lee CR,Lim LH

更新日期：1996-12-01 00:00:00

abstract：:Since the publication by Marmarou of a new rodent model of head injury, an increasing number of centres are adopting the model for use in a variety of paradigms. We report on two simple methods we have applied which allows us to monitor weight drop velocity and foam stiffness, both of which are critical factors for pr...

journal_title：Journal of neuroscience methods

authors： Piper IR,Thomson D,Miller JD

更新日期：1996-11-01 00:00:00

abstract：:Interactions among receptor neurons, glial cells and neurons intrinsic to the antennal lobe of the moth underlie the formation of olfactory glomeruli. To isolate these interactions, as well as to understand the effect of a variety of humoral agents on differentiation of the neurons and glia, we generate primary cultur...

journal_title：Journal of neuroscience methods

authors： Oland LA,Müller T,Kettenmann H,Hayashi J

更新日期：1996-10-21 00:00:00

abstract：:Although scalp potential distributions do not uniquely determine the location and configuration of neural generators, they are important because they provide the necessary conditions that any hypothesized sources must satisfy and suggest a basis for testing alternate source hypotheses. One problem that could confound ...

journal_title：Journal of neuroscience methods

authors： Sidman RD,Semel JS,Lagerlund TD,Ford MR

更新日期：1996-10-01 00:00:00

abstract：:We have developed a quantitative reverse transcription polymerase chain reaction (RT-PCR) method, using both internal and external standardisation, to quantitate fg amounts of pre-pro-thyrotropin releasing hormone (pre-pro-TRH) mRNA in the paraventricular nucleus (PVN) of individual laboratory rats. A constant amount ...

journal_title：Journal of neuroscience methods

authors： Muller LM,Kennedy JA

更新日期：1996-10-01 00:00:00

abstract：:An important parameter often investigated in the characterization of cortical functional organization is the areal extent of functional modules. Because it allows the visualization of functional modules with high spatial resolution in a noninvasive way to the cortex, intrinsic signal optical imaging (ISI) can be emplo...

journal_title：Journal of neuroscience methods

authors： Chen-Bee CH,Kwon MC,Masino SA,Frostig RD

更新日期：1996-09-01 00:00:00

abstract：:The 2-deoxyglucose (2DG) histochemical method of Sokoloff et al. ((1977) J. Neurochem., 28: 897-916) has been widely used to map metabolic activity in the brain, but lacks sufficient resolution to view individual neurons. Modifications of the original method have achieved cellular resolution with emulsion autoradiogra...

journal_title：Journal of neuroscience methods

authors： McCasland JS

更新日期：1996-09-01 00:00:00

abstract：:Several neuronal tracing substances were applied to the cut ends of leech cephalic nerves and the resulting backfills into the subesophageal ganglion (sbEG) were mapped. A 12 h incubation in 3 kDa dextrans conjugated either to a fluorochrome or to biotin (subsequently tagged with peroxidase) was satisfactory. In separ...

journal_title：Journal of neuroscience methods

authors： Daberkow DP,Vaughan DK

更新日期：1996-08-01 00:00:00

abstract：:Incubation chambers for surviving brain slice preparations are in most cases designed to be stationary. For investigations on human brain tissue resected for the treatment of brain tumor or epilepsy, portable incubation chambers are needed in addition to stationary ones to allow transport of the slices between laborat...

journal_title：Journal of neuroscience methods

authors： Köhling R,Lücke A,Straub H,Speckmann EJ

更新日期：1996-08-01 00:00:00

abstract：:A new method to study the reactions to unexpected mechanical perturbations during human walking on a treadmill is presented. Perturbations consisted of an obstruction of the forward swinging foot during the early swing phase. These were caused by obstacles which were dropped on the treadmill in front of the subject. T...

journal_title：Journal of neuroscience methods

authors： Schillings AM,Van Wezel BM,Duysens J

更新日期：1996-07-01 00:00:00

abstract：:We have developed a novel double-labeling method to investigate multiple gene expression in single cells. The method relies on the use of a radioactive probe followed by a colorimetric probe. Unique to this method, the radioactive signal is first captured on an emulsion pre-coated slide, which totally separates it fro...

journal_title：Journal of neuroscience methods

authors： Chiu KP,Duca KA,Berman SA,Sullivan T,Bursztajn S

更新日期：1996-06-01 00:00:00

abstract：:Although the classical Golgi method is a powerful means for structural analysis of the brain, it is generally considered to be an unpredictable technique making anatomists wary of using it. Often, even when successful staining has occurred, deposits of silver chromate crystals on the surface of the tissue obscure exam...

journal_title：Journal of neuroscience methods

authors： Angulo A,Fernández E,Merchán JA,Molina M

更新日期：1996-05-01 00:00:00

abstract：:The techniques of two experimental surgical operations on the trigeminal nerve are described, namely, excision of the trigeminal ganglion (ganglionectomy) and division of the trigeminal root (rhizotomy), in the cat. These techniques have been developed with the specific aims of achieving the trigeminal lesion and also...

journal_title：Journal of neuroscience methods

authors： Sakas DE,Whittaker K,Abbasi KH,Zervas NT

更新日期：1996-04-01 00:00:00

abstract：:An intra-arterially perfused working heart-brainstem preparation (WH-BP) was developed to allow studies into functionally identified cardiovascular and respiratory neurones in an in vitro milieu. This report provides that first description of this preparation. Evidence is presented indicating that the WH-BP: (1) spont...

journal_title：Journal of neuroscience methods

authors： Paton JF

更新日期：1996-03-01 00:00:00

abstract：:Unlike certain other brain areas, notably the hippocampal formation, synaptic transmission in the cerebellum, a 3-dimensional anatomical structure, is no ideally studied in a 2-dimensional slice preparation. We describe a method for cutting a cerebellar slice that incorporates two planes of section: one sagittal (in t...

journal_title：Journal of neuroscience methods

authors： Garthwaite J,Batchelor AM

更新日期：1996-02-01 00:00:00

abstract：:The branching patterns of the proximal middle cerebral artery (MCA) and their influence on the infarct size following MCA occlusion in rats were studied. In the proximal part from the level of the olfactory tract, the MCA most often presented one surface branch extending anteriorly, with a variable number of surface b...

journal_title：Journal of neuroscience methods

authors： Niiro M,Simon RP,Kadota K,Asakura T

更新日期：1996-01-01 00:00:00

abstract：:A dimensionally accurate clear model of the human scala tympani has been produced to evaluate the insertion and position of clinically applied intracochlear electrodes for electrical stimulation. Replicates of the human scala tympani were made from low melting point metal alloy (LMA) and from polymethylmeth-acrylate (...

journal_title：Journal of neuroscience methods

authors： Rebscher SJ,Talbot N,Bruszewski W,Heilmann M,Brasell J,Merzenich MM

更新日期：1996-01-01 00:00:00

abstract：:This paper describes a new method for the analysis of ensemble coding in populations of receptor afferents. The method is based on principal component analysis (PCA) combined with algorithms for calculation of stimulus separation (discrimination). It allows a quantification of the ability of ensembles of afferents to ...

journal_title：Journal of neuroscience methods

authors： Johansson H,Bergenheim M,Djupsjöbacka M,Sjölander P

更新日期：1995-12-01 00:00:00

abstract：:A method for automatically analyzing cortical signals recorded with voltage-sensitive dyes and a photodiode array is described. First, a forward-backward non-linear filtering technique is used to eliminate the background noise and preserve the fast transients of the signals. Then the filtered signals are deconvoluted ...

journal_title：Journal of neuroscience methods

authors： Litaudon P,Girardeau-Montaut C,Girardeau-Montaut JP,Cattarelli M

更新日期：1995-12-01 00:00:00

abstract：:We have designed a special dynamometer for measuring mouse forelimb muscle strength and endurance. The device exploits a mouse's tendency to grasp a horizontal metal bar while suspended by its tail. A threshold value for the magnitude and duration of force that the mouse can exert is obtained by first allowing the ani...

journal_title：Journal of neuroscience methods

authors： Smith JP,Hicks PS,Ortiz LR,Martinez MJ,Mandler RN

更新日期：1995-11-01 00:00:00

abstract：:A solution is described for the acquisition on a personal computer of standard pulses derived from neuronal discharge, measurement of neuronal discharge times, real-time control of stimulus delivery based on specified inter-pulse interval conditions in the neuronal spike train, and on-line display and analysis of the ...

journal_title：Journal of neuroscience methods

authors： Nordstrom MA,Mapletoft EA,Miles TS

更新日期：1995-11-01 00:00:00

abstract：:In electrophysiological recording, a microcomputer-based analog-to-digital (A/D) card is an indispensable instrument for signal acquisition and analysis. In our studies, evoked responses sampled by our A/D card showed variation among different cycles. If several cycles had been averaged, the resultant waveform would h...

journal_title：Journal of neuroscience methods

authors： Jaw FS,Lin CW,Wang CY

更新日期：1995-11-01 00:00:00

abstract：:A light microscopical, non-fluorescent, retrograde double-labeling technique is described. Cholera toxin B-subunit (CTb) and a conjugate of wheatgerm agglutinin and bovine serum albumin coupled to 10 nm gold particles (gold-lectin) are both excellent retrograde tracers and, when visualized by means of immunohistochemi...

journal_title：Journal of neuroscience methods

authors： Ruigrok TJ,Teune TM,van der Burg J,Sabel-Goedknegt H

更新日期：1995-09-01 00:00:00

abstract：:Selective and simultaneous voltammetric analysis of catechols and indoles in vivo and in vitro has until now been feasible only by means of 'slow' scanning methods (scan speed in tens of seconds) such as differential pulse (DPV) and differential normal pulse voltammetry in conjunction with electrically and/or chemical...

journal_title：Journal of neuroscience methods

authors： Crespi F,England TG,Trist DG

更新日期：1995-09-01 00:00:00

abstract：:Cross-correlation experiments allow to obtain information about synaptic potentials in human motoneurons. However, recording cross-correlation responses of one motoneuron to transcranial magnetic and electrical peripheral nerve stimulation requires a considerable recording time when both responses are recorded consecu...

journal_title：Journal of neuroscience methods

authors： Awiszus F,Feistner H

更新日期：1995-09-01 00:00:00

abstract：:A 2-channel action-potential generator system was designed for use in testing neurophysiologic data acquisition/analysis systems. The system consists of a personal computer controlling an external hardware unit. This system is capable of generating 2 channels of simulated action potential (AP) waveshapes. The AP wavef...

journal_title：Journal of neuroscience methods

authors： Lisiecki RS,Voigt HF

更新日期：1995-08-01 00:00:00

abstract：:We have adapted techniques for studying the locomotion of tethered insects to analysis of rapid directional movements such as escape behavior. We describe here a computer-based motion tracking system that allows an animal to turn and run as rapidly as it does under free-ranging conditions, and that samples fast enough...

journal_title：Journal of neuroscience methods

authors： Ye S,Dowd JP,Comer CM

更新日期：1995-08-01 00:00:00

abstract：:The process of peripheral nerve regeneration has been modeled using 5 populations of mathematical variables to represent the biological activities of Wallerian degeneration, fibrin matrix development, Schwann cell activity, elongating neurites, and neovascularization. The mathematical model provided simulations of ner...

journal_title：Journal of neuroscience methods

authors： Podhajsky RJ,Myers RR

更新日期：1995-08-01 00:00:00

abstract：:A simple method which allows easy quantification of neuronal and glial sub-populations in primary neuronal tissue culture is described. The method takes advantage of the fact that the staining of neuronal and glia nuclei with Hoechst 33258 is distinctive and characteristic of the cell type. Double-staining experiments...

journal_title：Journal of neuroscience methods

authors： Tucker LM,Morton AJ

更新日期：1995-07-01 00:00:00

abstract：:There are 3 known forms of synaptic plasticity at CNS synapses: long-term potentiation (LTP) mediated by NMDA receptor activation, LTP mediated by voltage-dependent calcium channel (VDCC) activation, and long-term depression (LTD) mediated by the NMDA receptor. All 3 forms of synaptic plasticity can be observed in hip...

journal_title：Journal of neuroscience methods

authors： Teyler TJ,Cavus I,Coussens C

更新日期：1995-06-01 00:00:00

abstract：:The recent emergence of surgical treatment of childhood epilepsy has led to the accessibility of young human cerebral tissue for electrophysiological studies of the mechanisms involved in epileptogenesis. Intracellular recordings were obtained from neurons in slices prepared from neocortical tissue resected from child...

journal_title：Journal of neuroscience methods

authors： Dudek FE,Wuarin JP,Tasker JG,Kim YI,Peacock WJ

更新日期：1995-06-01 00:00:00

abstract：:Behavioral recovery is the major goal of neuroscience research on the peripheral nervous system. However, behavioral methods of assessing peripheral nerve regeneration are still lacking. In the present work we used the median nerve and finger flexors as the experimental model. After resection or crush injury of the me...

journal_title：Journal of neuroscience methods

authors： Bertelli JA,Mira JC

更新日期：1995-05-01 00:00:00

abstract：:We describe a simplified HPLC method for the measurement of cocaine and catecholamines in the same brain tissue sample. Using this method to measure cocaine in the brain of rats which received either 10 or 20 mg/kg cocaine injections, we found substantial cocaine concentration differences between medial prefrontal cor...

journal_title：Journal of neuroscience methods

authors： Carey RJ,DePalma G

更新日期：1995-05-01 00:00:00